Oxford Harlequins RFC was formed in 1996 following the merger of two of the city's oldest rugby clubs, Oxford Marathons RFC and Oxford Old Boys RFC. The new club took the name Oxford Harlequins and adopted the colours of the "Marras" and the "Old Boys" into a new shirt with a classic harlequins quadrant design.

From 1996 to 2018 the club had several homes, starting out at Marston Ferry Road, the former home of Oxford Old Boys, before moving to the Marathons ground at Horspath, then to a ground-sharing agreement with Oxford RFC, before moving back to Marston Ferry Road for the 2016/17 season and 2017/18 season.

Finally, in 2018 a deal was struck between Oxford City Council and the RFU to grant Oxford Harlequins a long lease at Horspath Sports Ground with the club having tenure of all the playing fields, the clubhouse and the changing room blocks. The changing room block was refurbished at a cost of over £100,000, the clubhouse was remodelled and redecorated throughout and the new 3g pitch was built by the RFU at a cost of over £750,000. The result is a world-class rugby facility that will serve the city of Oxford for decades to come, the Oxford Rugby Hub, the new home of Oxford Harlequins.

Oxford Harlequins is now the largest and highest ranked rugby club in the city of Oxford and its environs, with the 1st XV playing in the Wadsworth South West Division, the 2nd XV playing in BBO Div 1 and our U18 Academy competing in the Ox Bucks and Berks U18 leagues and the National Under 18 Cup. We also have a great University Students / Under 20s section that play fixtures throughout the season against other student teams. Our over 35s play in the Oxfordshire Vets compeition with players involved from age 35 to 70. We have a thriving youth section, the largest in Oxford, with over 420 registered youth players and represenation at every age group from Under 6 to Under 18. Players are welcome to join the club at any age group.